Aspects of industrial purification of peptides using large-scale chromatography

نویسنده

  • Jonas Persson
چکیده

For purification of peptides, it is often difficult to use methods similar to those applied in the purification of other organic compounds, mainly due to their complexity. Purification of organic molecules often uses methods based on crystallization to isolate the desired molecule. As efficiency and high yields are of vital importance for optimal economy of any industrial manufacturing process, methods other than those based on crystallization have been explored for purification of peptides and peptide like molecules. These methods usually utilize various principles of chromatography such as ion exchange chromatography, gel permeation chromatography and mediumor high-pressure reversed phase chromatography. The examples mentioned are the most commonly used in peptide purification today but other methods have been used in the past, most noteworthy being counter current distribution [4] and partition chromatography [5].

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تاریخ انتشار 2004